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APOBEC3A Recombinant antibody, PBS Only (Detector)

APOBEC3A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241529G4

Cat no : 84239-4-PBS

Print datasheet

Synonyms

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

验证数据展示

  • WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2, APOBEC3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84239-1-PBS. Detection antibody: 84239-4-PBS. Standard: Ag18845. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84239-4-PBS

    Affinity and Kinetic Characterization of 84239-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 84239-4-PBS against Human APOBEC3A was performed. The affinity constant is 0.471 nM.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

产品信息

84239-4-PBS targets APOBEC3A as part of a matched antibody pair:

MP01122-2: 84239-1-PBS capture and 84239-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen APOBEC3A fusion protein Ag18845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Synonyms PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A
Calculated Molecular Weight 199 aa, 23 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC126416
Gene Symbol APOBEC3A
Gene ID (NCBI) 200315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31941
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.