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验证数据展示

  • WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    WB analysis using 84239-4-RR (same clone as 84239-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    FC experiment of A431 using 84239-4-RR (same clone as 84239-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84239-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2

    Cytometric bead array standard curve of MP01122-2, APOBEC3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84239-1-PBS. Detection antibody: 84239-4-PBS. Standard: Ag18845. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84239-4-PBS

    Affinity and Kinetic Characterization of 84239-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 84239-4-PBS against Human APOBEC3A was performed. The affinity constant is 0.471 nM.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

APOBEC3A Recombinant antibody, PBS Only (Detector)

APOBEC3A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84239-4-PBS

产品说明书

CloneNo. 241529G4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84239-4-PBS targets APOBEC3A as part of a matched antibody pair:

MP01122-2: 84239-1-PBS capture and 84239-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 APOBEC3A fusion protein Ag18845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
别名 PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A
计算分子量 199 aa, 23 kDa
观测分子量28 kDa
GenBank蛋白编号BC126416
基因名称 APOBEC3A
Gene ID (NCBI) 200315
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31941
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.