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验证数据展示

  • WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1, ASUN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84252-4-PBS. Detection antibody: 84252-3-PBS. Standard: Ag13727. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

ASUN Recombinant antibody, PBS Only (Capture)

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84252-4-PBS

产品说明书

CloneNo. 241586E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.

产品信息

84252-4-PBS targets ASUN as part of a matched antibody pair:

MP01142-1: 84252-4-PBS capture and 84252-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ASUN fusion protein Ag13727 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 11
别名 INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11
计算分子量 706 aa, 80 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC003081
基因名称 ASUN
Gene ID (NCBI) 55726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVM9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).