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ASUN Recombinant antibody, PBS Only (Capture)

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241586E9

Cat no : 84252-4-PBS

Print datasheet

Synonyms

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

验证数据展示

  • WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1, C12orf11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84252-4-PBS. Detection antibody: 84252-3-PBS. Standard: Ag13727. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.

产品信息

84252-4-PBS targets ASUN as part of a matched antibody pair:

MP01142-1: 84252-4-PBS capture and 84252-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ASUN fusion protein Ag13727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 12 open reading frame 11
Synonyms INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11
Calculated Molecular Weight 706 aa, 80 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC003081
Gene Symbol ASUN
Gene ID (NCBI) 55726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVM9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).