• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HCT 116 using 84292-4-RR

    FC experiment of HCT 116 using 84292-4-RR

    1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 84292-4-RR

    WB analysis using 84292-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84292-4-RR

    Affinity and Kinetic Characterization of 84292-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

ADAM17 Recombinant antibody

ADAM17 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84292-4-RR

产品说明书

CloneNo. 241305A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells, HCT 116 cells, HUVEC cells, SW480 cells, HeLa cells, RAW 264.7 cells
Positive FC (Intra) detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy ADAM17 Ready-To-Use IHC Kit. ADAM17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84292-4-RR targets ADAM17 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 ADAM17 fusion protein Ag32418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADAM metallopeptidase domain 17
别名 241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86
计算分子量 824 aa, 93 kDa
GenBank蛋白编号BC136783
基因名称 ADAM17
Gene ID (NCBI) 6868
RRIDAB_3671836
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

实验方案

Product Specific Protocols
WB protocol for ADAM17 antibody 84292-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols