验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, HuH-7 cells, HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ABCB1 Ready-To-Use IHC Kit. ABCB1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84295-3-RR targets P glycoprotein in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
| 别名 | ABCB1, 241512A1, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1 |
| 计算分子量 | 1280 aa, 142 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | BC130424 |
| 基因名称 | P glycoprotein |
| Gene ID (NCBI) | 5243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P08183 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P glycoprotein antibody 84295-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |