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验证数据展示

  • WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01211-2

    Sandwich ELISA standard curve of MP01211-2

    Sandwich ELISA standard curve of MP01211-2, Human ALKBH3 Recombinant Matched Antibody Pair - PBS only. 84314-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2982. 84314-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01211-2

    Sample test of MP01211-2

    The mean ALKBH3 concentration was determined to be 6.80 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load.

  • FC experiment of HeLa using 84314-2-RR (same clone as 84314-2-PBS)

    FC experiment of HeLa using 84314-2-RR (same clone as 84314-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 84314-2-RR (same clone as 84314-2-PBS)

    FC experiment of PC-3 using 84314-2-RR (same clone as 84314-2-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01211-1

    Cytometric bead array standard curve of MP01211-1

    Cytometric bead array standard curve of MP01211-1, ALKBH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84314-2-PBS. Detection antibody: 84314-1-PBS. Standard: Ag2982. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84314-2-PBS

    Affinity and Kinetic Characterization of 84314-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84314-2-PBS against Human ALKBH3 was performed. The affinity constant is 0.59 nM.

  • Affinity and Kinetic Characterization of 84314-2-RR

    Affinity and Kinetic Characterization of 84314-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84314-2-RR against Human ALKBH3 were performed. The affinity constant is 1.99 nM.

ALKBH3 Recombinant antibody, PBS Only (Capture)

ALKBH3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84314-2-PBS

产品说明书

CloneNo. 241650F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

产品信息

84314-2-PBS targets ALKBH3 as part of a matched antibody pair:

MP01211-1: 84314-2-PBS capture and 84314-1-PBS detection (validated in Cytometric bead array)

MP01211-2: 84314-2-PBS capture and 84314-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 ALKBH3 fusion protein Ag2982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkB, alkylation repair homolog 3 (E. coli)
别名 DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3
计算分子量 286 aa, 33 kDa
观测分子量30-33 kDa
GenBank蛋白编号BC015155
基因名称 ALKBH3
Gene ID (NCBI) 221120
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96Q83
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.