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HNF4A Recombinant antibody

HNF4A Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241593A2

Cat no : 84316-7-RR

Print datasheet

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

验证数据展示

  • IF Staining of HepG2 using 84316-7-RR

    IF Staining of HepG2 using 84316-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84316-7-RR

    IF Staining of HeLa using 84316-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84316-7-RR

    Affinity and Kinetic Characterization of 84316-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84316-7-RR targets HNF4A in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HNF4A fusion protein Ag24647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatocyte nuclear factor 4, alpha
Synonyms HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha
Calculated Molecular Weight 53 kDa
GenBank Accession NumberBC137539
Gene Symbol HNF4A
Gene ID (NCBI) 3172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP41235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).

实验方案

Product Specific Protocols
IF protocol for HNF4A antibody 84316-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols