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HNF4A Recombinant antibody, PBS Only

HNF4A Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241593A2

Cat no : 84316-7-PBS

Print datasheet

Synonyms

HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha

验证数据展示

  • IF Staining of HepG2 using 84316-7-RR (same clone as 84316-7-PBS)

    IF Staining of HepG2 using 84316-7-RR (same clone as 84316-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84316-7-RR (same clone as 84316-7-PBS)

    IF Staining of HeLa using 84316-7-RR (same clone as 84316-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84316-7-RR

    Affinity and Kinetic Characterization of 84316-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

产品信息

84316-7-PBS targets HNF4A in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen HNF4A fusion protein Ag24647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatocyte nuclear factor 4, alpha
Synonyms HNF4a8, HNF4a7, HNF4, HNF 4 alpha, Hepatocyte nuclear factor 4-alpha
Calculated Molecular Weight 53 kDa
GenBank Accession NumberBC137539
Gene Symbol HNF4A
Gene ID (NCBI) 3172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP41235
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).