• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SEL1L Recombinant antibody

SEL1L Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241594G2

Cat no : 84317-7-RR

Print datasheet

Synonyms

Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1

验证数据展示

  • WB analysis using 84317-7-RR

    WB analysis using 84317-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 84317-7-RR

    FC experiment of MCF-7 using 84317-7-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84317-7-RR

    Affinity and Kinetic Characterization of 84317-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, NIH/3T3 cells, mouse kidney tissue, rat liver tissue
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SEL1L fusion protein Ag32178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sel-1 suppressor of lin-12-like (C. elegans)
Synonyms Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1
Calculated Molecular Weight 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberNM_005065
Gene Symbol SEL1L
Gene ID (NCBI) 6400
RRIDAB_3671858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .

实验方案

Product Specific Protocols
WB protocol for SEL1L antibody 84317-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols