• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84317-7-RR (same clone as 84317-7-PBS)

    WB analysis using 84317-7-RR (same clone as 84317-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84317-7-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84317-7-RR (same clone as 84317-7-PBS)

    FC experiment of MCF-7 using 84317-7-RR (same clone as 84317-7-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84317-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84317-7-RR

    Affinity and Kinetic Characterization of 84317-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

SEL1L Recombinant antibody, PBS Only

SEL1L Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84317-7-PBS

Print datasheet

CloneNo. 241594G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84317-7-PBS targets SEL1L in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SEL1L fusion protein Ag32178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sel-1 suppressor of lin-12-like (C. elegans)
Synonyms Suppressor of lin-12-like protein 1, Sel-1L, Sel 1L, Protein sel-1 homolog 1, Protein sel 1 homolog 1
Calculated Molecular Weight 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberNM_005065
Gene Symbol SEL1L
Gene ID (NCBI) 6400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBV2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .