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验证数据展示

  • WB analysis using 84317-7-RR

    WB analysis using 84317-7-RR

    Mouse kidney tissue, rat liver tissue were subjected to SDS PAGE followed by western blot with 84317-7-RR (SEL1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 84317-7-RR

    FC experiment of MCF-7 using 84317-7-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SEL1L Recombinant antibody (84317-7-RR, Clone:241594G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84317-7-RR

    Affinity and Kinetic Characterization of 84317-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84317-7-RR against Human SEL1L were performed. The affinity constant is 6.03 nM.

SEL1L Recombinant antibody

SEL1L Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84317-7-RR

产品说明书

CloneNo. 241594G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

241594G2, Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, U-87 MG cells, mouse kidney tissue, rat liver tissue
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84317-7-RR targets SEL1L in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEL1L fusion protein Ag32178 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sel-1 suppressor of lin-12-like (C. elegans)
别名 241594G2, Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L
计算分子量 89 kDa
观测分子量89 kDa
GenBank蛋白编号NM_005065
基因名称 SEL1L
Gene ID (NCBI) 6400
RRIDAB_3671858
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEL1L (Suppressor Enhancer Lin-12 Like) is an endoplasmic reticulum-resident transmembrane protein that plays a critical role in collagen metabolism. It has been identified as an intracellular rheostat controlling collagen turnover, which is the balance between collagen synthesis and degradation. The protein's luminal face contains a Fibronectin-2 (FN2) domain, which is known to bind collagen. SEL1L functions as a sensor of collagen biosynthesis and is necessary for the upregulation of MRC2 (mannose receptor, C-type 2), a collagen endocytic receptor that mediates the clearance of collagen. .

实验方案

Product Specific Protocols
WB protocol for SEL1L antibody 84317-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols