验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84353-1-RR targets SLCO2B1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier organic anion transporter family, member 2B1 |
| 别名 | OATPRP2, OATPB, OATP2B1, OATP B, KIAA0880 |
| 计算分子量 | 77 kDa |
| 观测分子量 | 61~74 kDa |
| GenBank蛋白编号 | NM_007256 |
| 基因名称 | SLCO2B1 |
| Gene ID (NCBI) | 11309 |
| RRID | AB_3671891 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O94956 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Solute carrier organic anion (SLCO) gene families encode organic anion transport proteins (OATP), which are membrane transporters widely expressed in the human body that influx numerous compounds and drugs including androgens. SLCO2B1 is a member of an organic anion transporter family and has previously been shown to transport a multitude of drugs and steroid hormone conjugates (PMID: 35714613). High expression of SLCO2B1 is known to associate with the resistance to androgen deprivation therapy in prostate cancer (PMID: 29581838). OATP2B1 was insensitive to endoglycosidase H, an enzyme that cleaves only high mannose glycans, as expected for a mature protein undergoing complex glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLCO2B1 antibody 84353-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |