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验证数据展示

  • WB analysis of rat skeletal muscle using 84356-1-RR

    WB analysis of rat skeletal muscle using 84356-1-RR

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 84356-1-RR

    IF Staining of mouse skeletal muscle using 84356-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse skeletal muscle using 84356-1-RR

    IHC staining of mouse skeletal muscle using 84356-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84356-1-RR

    Affinity and Kinetic Characterization of 84356-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

ACTN3 Recombinant antibody

ACTN3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84356-1-RR

产品说明书

CloneNo. 241168A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84356-1-RR targets ACTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 ACTN3 fusion protein Ag36709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actinin, alpha 3
别名 Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3
计算分子量 901 aa, 103 kDa
观测分子量100 kDa
GenBank蛋白编号BC099647
基因名称 ACTN3
Gene ID (NCBI) 89
RRIDAB_3671894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ08043
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTN3 antibody 84356-1-RRDownload protocol
IHC protocol for ACTN3 antibody 84356-1-RRDownload protocol
IF protocol for ACTN3 antibody 84356-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols