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验证数据展示

  • WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84356-1-RR

    Affinity and Kinetic Characterization of 84356-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

ACTN3 Recombinant antibody, PBS Only

ACTN3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84356-1-PBS

产品说明书

CloneNo. 241168A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84356-1-PBS targets ACTN3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 ACTN3 fusion protein Ag36709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actinin, alpha 3
别名 Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3
计算分子量 901 aa, 103 kDa
观测分子量100 kDa
GenBank蛋白编号BC099647
基因名称 ACTN3
Gene ID (NCBI) 89
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ08043
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C