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验证数据展示

  • WB analysis of mouse spleen using 84365-5-RR

    WB analysis of mouse spleen using 84365-5-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 84365-5-RR

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84365-5-RR

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 84365-5-RR

    IF Staining of mouse spleen using 84365-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SIGNR1/CD209b antibody (84365-5-RR, Clone: 241726B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84365-5-RR

    Affinity and Kinetic Characterization of 84365-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84365-5-RR against Mouse SIGNR1/CD209b were performed. The affinity constant is 0.155 nM.

SIGNR1/CD209b Recombinant monoclonal antibody

SIGNR1/CD209b Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84365-5-RR

产品说明书

CloneNo. 241726B1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84365-5-RR targets SIGNR1/CD209b in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1497

Product name: Recombinant Mouse SIGNR1/CD209b protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 74-325 aa of NM_026972.5

Sequence: QVSKTPNTERQKEQEKILQELTQLTDELTSRIPISQGKNESMQAKITEQLMQLKTELLSRIPIFQGQNESIQEKISEQLMQLKAELLSKISSFPVKDDSKQEKIYQQLVQMKTELFRLCRLCPWDWTFLLGNCYFFSKSQRNWNDAVTACKEVKAQLVIINSDEEQTFLQQTSKAKGPTWMGLSDLKKEATWLWVDGSTLSSRFQKYWNRGEPNNIGEEDCVEFAGDGWNDSKCELKKFWICKKSATPCTEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD209b antigen
别名 241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1
计算分子量37 kDa
观测分子量45-50 kDa
GenBank蛋白编号NM_026972.5
基因名称 Cd209b
Gene ID (NCBI) 69165
RRIDAB_3671901
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8CJ91-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor (PMID: 11581173). SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain (PMID: 15583012). It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.

实验方案

Product Specific Protocols
IF protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
IHC protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
WB protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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