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验证数据展示

  • WB analysis of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    WB analysis of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84365-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    IHC staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84365-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    IHC staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84365-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    IF Staining of mouse spleen using 84365-5-RR (same clone as 84365-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SIGNR1/CD209b antibody (84365-5-RR, Clone: 241726B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84365-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84365-5-RR

    Affinity and Kinetic Characterization of 84365-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84365-5-RR against Mouse SIGNR1/CD209b were performed. The affinity constant is 0.155 nM.

Mouse SIGNR1/CD209b Recombinant monoclonal antibody, PBS Only

SIGNR1/CD209b Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84365-5-PBS

产品说明书

CloneNo. 241726B1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84365-5-PBS targets SIGNR1/CD209b in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1497

Product name: Recombinant Mouse SIGNR1/CD209b protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 74-325 aa of NM_026972.5

Sequence: QVSKTPNTERQKEQEKILQELTQLTDELTSRIPISQGKNESMQAKITEQLMQLKTELLSRIPIFQGQNESIQEKISEQLMQLKAELLSKISSFPVKDDSKQEKIYQQLVQMKTELFRLCRLCPWDWTFLLGNCYFFSKSQRNWNDAVTACKEVKAQLVIINSDEEQTFLQQTSKAKGPTWMGLSDLKKEATWLWVDGSTLSSRFQKYWNRGEPNNIGEEDCVEFAGDGWNDSKCELKKFWICKKSATPCTEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD209b antigen
别名 241726B1, CD209, CD209 antigen-like protein B, Cd209b, DC-SIGNR1
计算分子量37 kDa
观测分子量45-50 kDa
GenBank蛋白编号NM_026972.5
基因名称 Cd209b
Gene ID (NCBI) 69165
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8CJ91-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor (PMID: 11581173). SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain (PMID: 15583012). It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.

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