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验证数据展示

  • WB analysis using 84373-6-RR

    WB analysis using 84373-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84373-6-RR (THAP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84373-6-RR

    IF Staining of HeLa using 84373-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THAP11 antibody (84373-6-RR, Clone: 241590E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 84373-6-RR

    FC experiment of HepG2 using 84373-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug THAP11 Recombinant antibody (84373-6-RR, Clone:241590E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84373-6-RR

    Affinity and Kinetic Characterization of 84373-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84373-6-RR against Human THAP11 were performed. The affinity constant is below 1 pM.

THAP11 Recombinant antibody

THAP11 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84373-6-RR

产品说明书

CloneNo. 241590E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, NCCIT cells, HT-29 cells, C2C12 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy THAP11 Ready-To-Use IHC Kit. THAP11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84373-6-RR targets THAP11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 THAP11 fusion protein Ag19342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 THAP domain containing 11
别名 THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a
计算分子量 314 aa, 34 kDa
观测分子量45 kDa
GenBank蛋白编号BC012182
基因名称 THAP11
Gene ID (NCBI) 57215
RRIDAB_3671909
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96EK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THAP11 antibody 84373-6-RRDownload protocol
IF protocol for THAP11 antibody 84373-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols