• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody

Phospho-IkB Beta (Thr19/Ser23) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241061B2

Cat no : 84403-1-RR

Print datasheet

Synonyms

IkB beta, IκB beta, IκB β, NFKBIB, IkB B

验证数据展示

  • WB analysis of HT-29 using 84403-1-RR

    WB analysis of HT-29 using 84403-1-RR

    Non-treated HT-29 cells and TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84403-1-RR (Phospho-IkB Beta (Thr19/Ser23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • FC experiment of HT-29 using 84403-1-RR

    FC experiment of HT-29 using 84403-1-RR

    1x10^6 TNF alpha and Calyculin A treated or untreated(black) HT-29 cells were intracellularly stained with 0.25 ug Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody (84403-1-RR, Clone:241061B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inTNF alpha and Calyculin A treated HT-29 cells
Positive FC (Intra) detected inTNF alpha and Calyculin A treated HT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84403-1-RR targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Synonyms IkB beta, IκB beta, IκB β, NFKBIB, IkB B
Calculated Molecular Weight 356 aa, 38 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC015528
Gene Symbol IkB Beta
Gene ID (NCBI) 4793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.

实验方案

Product Specific Protocols
WB protocol for Phospho-IkB Beta (Thr19/Ser23) antibody 84403-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols