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验证数据展示

  • WB analysis of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    WB analysis of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    Non-treated HT-29 cells and TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84403-1-RR (Phospho-IkB Beta (Thr19/Ser23) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84403-1-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    FC experiment of HT-29 using 84403-1-RR (same clone as 84403-1-PBS)

    1x10^6 TNF alpha and Calyculin A treated or untreated(black) HT-29 cells were intracellularly stained with 0.25 ug Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody (84403-1-RR, Clone:241061B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84403-1-PBS in a different storage buffer formulation.

Phospho-IkB Beta (Thr19/Ser23) Recombinant antibody, PBS Only

Phospho-IkB Beta (Thr19/Ser23) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84403-1-PBS

产品说明书

CloneNo. 241061B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

IkB beta, IκB beta, IκB β, NFKBIB, IkB B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84403-1-PBS targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
别名 IkB beta, IκB beta, IκB β, NFKBIB, IkB B
计算分子量 356 aa, 38 kDa
观测分子量 50 kDa
GenBank蛋白编号BC015528
基因名称 IkB Beta
Gene ID (NCBI) 4793
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15653
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.