验证数据展示
经过测试的应用
| Positive WB detected in | TNF alpha and Calyculin A treated HT-29 cells |
| Positive FC (Intra) detected in | TNF alpha and Calyculin A treated HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84403-1-RR targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta |
| 别名 | IkB beta, IκB beta, IκB β, NFKBIB, IkB B |
| 计算分子量 | 356 aa, 38 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC015528 |
| 基因名称 | IkB Beta |
| Gene ID (NCBI) | 4793 |
| RRID | AB_3671932 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q15653 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-IkB Beta (Thr19/Ser23) antibody 84403-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |