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验证数据展示

  • WB analysis using 84407-4-RR

    WB analysis using 84407-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84407-4-RR (ACE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat lung using 84407-4-RR

    IHC staining of rat lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 84407-4-RR

    IHC staining of mouse lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 84407-4-RR

    IHC staining of mouse lung using 84407-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACE Recombinant antibody

ACE Recombinant Antibody for WB, IHC, ELISA
Cat No. 84407-4-RR

产品说明书

CloneNo. 241763E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme, ACE1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84407-4-RR targets ACE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
别名 DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme, ACE1
计算分子量150kDa
观测分子量170-180 kDa
GenBank蛋白编号NM_000789.4
基因名称 ACE
Gene ID (NCBI) 1636
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP12821-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

实验方案

Product Specific Protocols
WB protocol for ACE antibody 84407-4-RRDownload protocol
IHC protocol for ACE antibody 84407-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols