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验证数据展示

  • WB analysis using 84407-4-RR (same clone as 84407-4-PBS)

    WB analysis using 84407-4-RR (same clone as 84407-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84407-4-RR (ACE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84407-4-PBS in a different storage buffer formulation.

  • IHC staining of rat lung using 84407-4-RR (same clone as 84407-4-PBS)

    IHC staining of rat lung using 84407-4-RR (same clone as 84407-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84407-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 84407-4-RR (same clone as 84407-4-PBS)

    IHC staining of mouse lung using 84407-4-RR (same clone as 84407-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84407-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 84407-4-RR (same clone as 84407-4-PBS)

    IHC staining of mouse lung using 84407-4-RR (same clone as 84407-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84407-4-RR (ACE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84407-4-PBS in a different storage buffer formulation.

ACE Recombinant antibody, PBS Only

ACE Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84407-4-PBS

产品说明书

CloneNo. 241763E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme, ACE1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84407-4-PBS targets ACE in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
别名 DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme, ACE1
计算分子量150kDa
观测分子量170-180 kDa
GenBank蛋白编号NM_000789.4
基因名称 ACE
Gene ID (NCBI) 1636
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP12821-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).