• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IL-4 Recombinant antibody

IL-4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241249H7

Cat no : 84422-5-RR

Print datasheet

Synonyms

Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1

验证数据展示

  • WB analysis of Recombinant protein using 84422-5-RR

    WB analysis of Recombinant protein using 84422-5-RR

    Rat IL-4 recombinant protein (Cat No. Eg113) was subjected to SDS PAGE followed by western blot with 84422-5-RR (IL-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84422-5-RR targets IL-4 in WB, ELISA applications and shows reactivity with rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 4
Synonyms Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1
Calculated Molecular Weight16kDa
GenBank Accession NumberNM_201270.1
Gene Symbol IL-4
Gene ID (NCBI) 287287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20096
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.

实验方案

Product Specific Protocols
WB protocol for IL-4 antibody 84422-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols