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Rat IL-4 Recombinant antibody, PBS Only (Capture/Detector)

IL-4 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241249H7

Cat no : 84422-5-PBS

Print datasheet

Synonyms

Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1

验证数据展示

  • WB analysis of Recombinant protein using 84422-5-RR (same clone as 84422-5-PBS)

    WB analysis of Recombinant protein using 84422-5-RR (same clone as 84422-5-PBS)

    Rat IL-4 recombinant protein (Cat No. Eg113) was subjected to SDS PAGE followed by western blot with 84422-5-RR (IL-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84422-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

  • Cytometric bead array standard curve of MP01293-3

    Cytometric bead array standard curve of MP01293-3

    Cytometric bead array standard curve of MP01293-3, RAT IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84422-5-PBS. Detection antibody: 84422-2-PBS. Standard: Eg1113. Range: 1.25-80 ng/mL.

产品信息

84422-5-PBS targets IL-4 as part of a matched antibody pair:

MP01293-3: 84422-5-PBS capture and 84422-2-PBS detection (validated in Cytometric bead array)

MP01293-4: 84422-3-PBS capture and 84422-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 4
Synonyms Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1
Calculated Molecular Weight16kDa
GenBank Accession NumberNM_201270.1
Gene Symbol IL-4
Gene ID (NCBI) 287287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20096
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.