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验证数据展示

  • WB analysis of mouse brain using 84434-4-RR

    WB analysis of mouse brain using 84434-4-RR

    mouse brain tissue was subjected to SDS PAGE followed by western blot with 84434-4-RR (5'-Nucleotidase/CD73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of 4T1 using 84434-4-RR

    IF Staining of 4T1 using 84434-4-RR

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using 5'-Nucleotidase/CD73 antibody (84434-4-RR, Clone: 241541F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84434-4-RR

    Affinity and Kinetic Characterization of 84434-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84434-4-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

CD73 Recombinant antibody

CD73 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84434-4-RR

产品说明书

CloneNo. 241541F5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected in4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84434-4-RR targets CD73 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5' nucleotidase, ecto
别名 Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
计算分子量64kDa
观测分子量60 kDa
GenBank蛋白编号NM_011851.4
基因名称 Nt5e
Gene ID (NCBI) 23959
RRIDAB_3671960
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ61503
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
WB protocol for CD73 antibody 84434-4-RRDownload protocol
IF protocol for CD73 antibody 84434-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols