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验证数据展示

  • WB analysis of mouse brain using 84434-4-RR (same clone as 84434-4-PBS)

    WB analysis of mouse brain using 84434-4-RR (same clone as 84434-4-PBS)

    mouse brain tissue was subjected to SDS PAGE followed by western blot with 84434-4-RR (5'-Nucleotidase/CD73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • IF Staining of 4T1 using 84434-4-RR (same clone as 84434-4-PBS)

    IF Staining of 4T1 using 84434-4-RR (same clone as 84434-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using 5'-Nucleotidase/CD73 antibody (84434-4-RR, Clone: 241541F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01279-1

    Cytometric bead array standard curve of MP01279-1

    Cytometric bead array standard curve of MP01279-1, MOUSE CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84434-3-PBS. Detection antibody: 84434-4-PBS. Standard: Eg1389. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84434-4-RR

    Affinity and Kinetic Characterization of 84434-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84434-4-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

Mouse CD73 Recombinant antibody, PBS Only (Detector)

CD73 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84434-4-PBS

产品说明书

CloneNo. 241541F5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84434-4-PBS targets CD73 as part of a matched antibody pair:

MP01279-1: 84434-3-PBS capture and 84434-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5' nucleotidase, ecto
别名 Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
计算分子量64kDa
观测分子量60 kDa
GenBank蛋白编号NM_011851.4
基因名称 Nt5e
Gene ID (NCBI) 23959
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ61503
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).