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Acetyl-Histone H2B (Lys16) Recombinant antibody

Acetyl-Histone H2B (Lys16) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241194H2

Cat no : 84446-1-RR

Print datasheet

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

验证数据展示

  • Dot Blot experiment of peptide using 84446-1-RR

    Dot Blot experiment of peptide using 84446-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys16) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis of NIH/3T3 using 84446-1-RR

    WB analysis of NIH/3T3 using 84446-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84446-1-RR (Histone H2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84446-1-RR

    IF Staining of HeLa using 84446-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H2B antibody (84446-1-RR, Clone: 241194H2 ) at dilution of 1:850 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:425-1:1700
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84446-1-RR targets Acetyl-Histone H2B (Lys16) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys16) antibody 84446-1-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys16) antibody 84446-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols