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验证数据展示

  • WB analysis using 84453-1-RR

    WB analysis using 84453-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84453-1-RR (TGFBR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84453-1-RR

    IHC staining of human placenta using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 84453-1-RR

    IHC staining of human lung cancer using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 84453-1-RR

    IHC staining of human lung cancer using 84453-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84453-1-RR

    Affinity and Kinetic Characterization of 84453-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84453-1-RR against Human TGFBR1 were performed. The affinity constant is 67.5 pM.

TGFBR1 Recombinant antibody

TGFBR1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84453-1-RR

产品说明书

CloneNo. 241441A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Activin receptor-like kinase 5, Activin receptor like kinase 5, Activin A receptor type II-like protein kinase of 53kD, AAT5, 241441A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, MDA-MB-231 cells, A549 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84453-1-RR targets TGFBR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TGFBR1 fusion protein Ag31620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta receptor 1
别名 Activin receptor-like kinase 5, Activin receptor like kinase 5, Activin A receptor type II-like protein kinase of 53kD, AAT5, 241441A11
计算分子量56KD
观测分子量56 kDa
GenBank蛋白编号NM_004612
基因名称 TGFBR1
Gene ID (NCBI) 7046
RRIDAB_3671979
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).

实验方案

Product Specific Protocols
WB protocol for TGFBR1 antibody 84453-1-RRDownload protocol
IHC protocol for TGFBR1 antibody 84453-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols