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VPS28 Recombinant antibody

VPS28 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241886D3

Cat no : 84472-4-RR

Print datasheet

Synonyms

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3

验证数据展示

  • WB analysis using 84472-4-RR

    WB analysis using 84472-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, THP-1 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84472-4-RR is VPS28 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen VPS28 fusion protein Ag7784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Synonyms Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UK41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

实验方案

Product Specific Protocols
WB protocol for VPS28 antibody 84472-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols