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验证数据展示

  • WB analysis using 84478-6-RR

    WB analysis using 84478-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84478-6-RR (FKBP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84478-6-RR

    WB analysis using 84478-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84478-6-RR (FKBP11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 84478-6-RR

    WB analysis of Raji using 84478-6-RR

    WB result of FKBP11 antibody (84478-6-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP11 transfected Raji cells.

  • Affinity and Kinetic Characterization of 84478-6-RR

    Affinity and Kinetic Characterization of 84478-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84478-6-RR against Human FKBP11 were performed. The affinity constant is 1.93 nM.

  • Featured Product
  • KD/KO验证

FKBP11 Recombinant monoclonal antibody

FKBP11 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84478-6-RR

产品说明书

CloneNo. 241912D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

19 kDa FK506 binding protein, 19 kDa FK506-binding protein, 19 kDa FKBP, 241912D2, EC:5.2.1.8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84478-6-RR targets FKBP11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11368

Product name: Recombinant human FKBP11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-201 aa of BC027973

Sequence: AEAGLETESPVRTLQVETLVEPPEPCAEPAAFGDTLHIHYTGSLVDGRIIDTSLTRDPLVIELGQKQVIPGLEQSLLDMCVGEKRRAIIPSHLAYGKRGFPPSVPADAVVQYDVELIALIRANYWLKLVKGILPLVGMAMVPALLGLIGYHLYRKANRPKVSKKKLKEEKRNKSKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FK506 binding protein 11, 19 kDa
别名 19 kDa FK506 binding protein, 19 kDa FK506-binding protein, 19 kDa FKBP, 241912D2, EC:5.2.1.8
计算分子量 201 aa, 22 kDa
观测分子量24 kDa
GenBank蛋白编号BC027973
基因名称 FKBP11
Gene ID (NCBI) 51303
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NYL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP11 (FK506 binding protein 11, also named FKBP19) was first described as predominantly expressed in secretory tissues including the pancreas and recent studies have suggested a role in beta cell survival under conditions of ER stress (PMID: 35456020). In addition, FKBP11 appears to play a role in osteoblasts and bone formation where it has been observed to associate with interferon-inducible transmembrane protein 5 (IFITM5) (PMID: 24058703).

实验方案

Product Specific Protocols
WB protocol for FKBP11 antibody 84478-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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