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验证数据展示

  • WB analysis using 84480-2-RR

    WB analysis using 84480-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84480-2-RR

    IF Staining of HeLa using 84480-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84480-2-RR

    FC experiment of HeLa using 84480-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GSR Recombinant antibody (84480-2-RR, Clone:241934D2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84480-2-RR

    Affinity and Kinetic Characterization of 84480-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

GSR Recombinant antibody

GSR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84480-2-RR

产品说明书

CloneNo. 241934D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HeLa cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84480-2-RR targets GSR in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GSR fusion protein Ag13080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione reductase
别名 GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7
计算分子量 522 aa, 56 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC069244
基因名称 GSR
Gene ID (NCBI) 2936
RRIDAB_3672000
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00390
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.

实验方案

Product Specific Protocols
WB protocol for GSR antibody 84480-2-RRDownload protocol
IF protocol for GSR antibody 84480-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols