验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293T cells, HuH-7 cells, HeLa cells, Jurkat cells, HepG2 cells, HCT 116 cells, U2OS cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy UHRF1 Ready-To-Use IHC Kit. UHRF1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84489-5-RR targets UHRF1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | UHRF1 fusion protein Ag17498 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ubiquitin-like with PHD and ring finger domains 1 |
| 别名 | 241872G4, E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1 |
| 计算分子量 | 806 aa, 91 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC113875 |
| 基因名称 | UHRF1 |
| Gene ID (NCBI) | 29128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q96T88 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UHRF1 antibody 84489-5-RR | Download protocol |
| IF protocol for UHRF1 antibody 84489-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |