• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84507-4-RR

    WB analysis using 84507-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84507-4-RR (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84507-4-RR

    IHC staining of human tonsillitis using 84507-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD63 antibody included
  • IF Staining of A431 using 84507-4-RR

    IF Staining of A431 using 84507-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD63 antibody (84507-4-RR, Clone: 241964H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human tonsillitis using 84507-4-RR

    IHC staining of human tonsillitis using 84507-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD63 antibody included

CD63 Recombinant antibody

CD63 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84507-4-RR

产品说明书

CloneNo. 241964H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84507-4-RR targets CD63 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD63 molecule
别名 Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63
计算分子量 26 kDa
观测分子量30-60 kDa
GenBank蛋白编号BC002349
基因名称 CD63
Gene ID (NCBI) 967
RRIDAB_3672016
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08962
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
WB protocol for CD63 antibody 84507-4-RRDownload protocol
IHC protocol for CD63 antibody 84507-4-RRDownload protocol
IF protocol for CD63 antibody 84507-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols