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验证数据展示

  • IF Staining of bEnd.3 using 84529-3-RR (same clone as 84529-3-PBS)

    IF Staining of bEnd.3 using 84529-3-RR (same clone as 84529-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84529-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01386-3

    Sandwich ELISA standard curve of MP01386-3

    Sandwich ELISA standard curve of MP01386-3, Mouse VE-cadherin/CD144 Recombinant Matched Antibody Pair - PBS only. 84529-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0921. 84529-6-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

  • Sample test of MP01386-3

    Sample test of MP01386-3

    The mean VE-cadherin/CD144 concentration was determined to be 3,858.35 ng/mL in mouse heart tissue extract based on a 1.20 mg/mL extract load and 709.13 ng/mL in bEnd.3 cell extract based on a 1.80 mg/mL extract load.

  • Cytometric bead array standard curve of MP01386-2

    Cytometric bead array standard curve of MP01386-2

    Cytometric bead array standard curve of MP01386-2, MOUSE VE-cadherin/CD144 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84529-3-PBS. Detection antibody: 84529-2-PBS. Standard: Eg0921. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84529-3-RR

    Affinity and Kinetic Characterization of 84529-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

Mouse VE-cadherin Recombinant antibody, PBS Only (Capture)

VE-cadherin Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84529-3-PBS

产品说明书

CloneNo. 241953H6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

241953H6, 7B4, cadherin 5, Cd144, Cdh5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84529-3-PBS targets VE-cadherin as part of a matched antibody pair:

MP01386-2: 84529-3-PBS capture and 84529-2-PBS detection (validated in Cytometric bead array)

MP01386-3: 84529-3-PBS capture and 84529-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 VE-cadherin fusion protein Eg0921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 5
别名 241953H6, 7B4, cadherin 5, Cd144, Cdh5
计算分子量88kDa
GenBank蛋白编号NM_009868.4
基因名称 Cdh5
Gene ID (NCBI) 12562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55284
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).