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VE-cadherin Recombinant antibody

VE-cadherin Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241953H6

Cat no : 84529-3-RR

Print datasheet

Synonyms

VE Cad, Cdh5, Cd144, cadherin 5, 7B4

验证数据展示

  • IF Staining of bEnd.3 using 84529-3-RR

    IF Staining of bEnd.3 using 84529-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using VE-cadherin antibody (84529-3-RR, Clone: 241953H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84529-3-RR

    Affinity and Kinetic Characterization of 84529-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84529-3-RR against Mouse VE-cadherin were performed. The affinity constant is 1.22 nM.

经过测试的应用

Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84529-3-RR targets VE-cadherin in IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 5
Synonyms VE Cad, Cdh5, Cd144, cadherin 5, 7B4
Calculated Molecular Weight88kDa
GenBank Accession NumberNM_009868.4
Gene Symbol Cdh5
Gene ID (NCBI) 12562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

实验方案

Product Specific Protocols
IF protocol for VE-cadherin antibody 84529-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols