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验证数据展示

  • WB analysis using 84534-5-RR

    WB analysis using 84534-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84534-5-RR

    IHC staining of human ovary cancer using 84534-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 84534-5-RR

    IHC staining of human ovary cancer using 84534-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84534-5-RR

    Affinity and Kinetic Characterization of 84534-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

MDMX/MDM4 Recombinant antibody

MDMX/MDM4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84534-5-RR

产品说明书

CloneNo. 241936D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293T cells, A549 cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84534-5-RR targets MDMX/MDM4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MDMX/MDM4 fusion protein Ag26678 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Mdm4 p53 binding protein homolog (mouse)
别名 MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX
计算分子量 490 aa, 55 kDa
观测分子量 70-76 kDa
GenBank蛋白编号BC067299
基因名称 MDMX/MDM4
Gene ID (NCBI) 4194
RRIDAB_3672041
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MDMX/MDM4 antibody 84534-5-RRDownload protocol
IHC protocol for MDMX/MDM4 antibody 84534-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols