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MDMX/MDM4 Recombinant antibody, PBS Only

MDMX/MDM4 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241936D11

Cat no : 84534-5-PBS

Print datasheet

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

验证数据展示

  • WB analysis using 84534-5-RR (same clone as 84534-5-PBS)

    WB analysis using 84534-5-RR (same clone as 84534-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

    IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

    IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84534-5-RR

    Affinity and Kinetic Characterization of 84534-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

产品信息

84534-5-PBS targets MDMX/MDM4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MDMX/MDM4 fusion protein Ag26678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Mdm4 p53 binding protein homolog (mouse)
Synonyms MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX
Calculated Molecular Weight 490 aa, 55 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC067299
Gene Symbol MDMX/MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15151
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.