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ZMPSTE24 Recombinant antibody

ZMPSTE24 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241288C11

Cat no : 84547-1-RR

Print datasheet

Synonyms

FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog

验证数据展示

  • WB analysis using 84547-1-RR

    WB analysis using 84547-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84547-1-RR (ZMPSTE24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84547-1-RR

    Affinity and Kinetic Characterization of 84547-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84547-1-RR against Human ZMPSTE24 were performed. The affinity constant is 1.87 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, PC-3 cells, SKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84547-1-RR is ZMPSTE24 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ZMPSTE24 fusion protein Ag33613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc metallopeptidase (STE24 homolog, S. cerevisiae)
Synonyms FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog
Calculated Molecular Weight 475 aa, 55 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC037283
Gene Symbol ZMPSTE24
Gene ID (NCBI) 10269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

实验方案

Product Specific Protocols
WB protocol for ZMPSTE24 antibody 84547-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols