WNT3A Recombinant antibody
WNT3A Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241993E8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84577-4-RR targets WNT3A in IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | WNT3A fusion protein Ag25051 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | wingless-type MMTV integration site family, member 3A |
| Synonyms | Protein Wnt-3a, Protein Wnt 3a, 241993E8 |
| Calculated Molecular Weight | 352 aa, 39 kDa |
| GenBank Accession Number | BC103921 |
| Gene Symbol | WNT3A |
| Gene ID (NCBI) | 89780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P56704 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for WNT3A antibody 84577-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |