CD99 Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP01403-3, Humam CD99 Recombinant Matched Antibody Pair - PBS only. 84580-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02321. 84580-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

The mean CD99 concentration was determined to be 328.33 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 96.35 ng/mL in A431 cell extract based on a 1.3 mg/mL extract load.

HEK-293 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate wasremoved, assayed for human CD99, and measured 1.59 ng/mL.

Urine of six individual healthy human donors was measured. The human CD99 concentration of detected samples was determined to be 93.60 ng/mL with a range of 19.59 - 184.77 ng/mL.