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验证数据展示

  • WB analysis using 84580-4-RR

    WB analysis using 84580-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84580-4-RR (CD99 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84580-4-RR

    IHC staining of human tonsillitis using 84580-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84580-4-RR

    IHC staining of human tonsillitis using 84580-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84580-4-RR

    IHC staining of human tonsillitis using 84580-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 84580-4-RR

    IF Staining of Jurkat using 84580-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD99 antibody (84580-4-RR, Clone: 241737H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD99 Recombinant antibody

CD99 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84580-4-RR

产品说明书

CloneNo. 241737H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, Jurkat cells, HUVEC cells, U-937 cells, U-251 cells, MOLT-4 cells, human testis tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84580-4-RR targets CD99 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD99 molecule
别名 MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1
计算分子量 19 kDa, 16 kDa, 17 kDa
观测分子量28 kDa, 32 kDa
GenBank蛋白编号BC021620
基因名称 CD99
Gene ID (NCBI) 4267
RRIDAB_3672079
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP14209
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

实验方案

Product Specific Protocols
WB protocol for CD99 antibody 84580-4-RRDownload protocol
IHC protocol for CD99 antibody 84580-4-RRDownload protocol
IF protocol for CD99 antibody 84580-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols