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CD99 Recombinant antibody, PBS Only

CD99 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241737H1

Cat no : 84580-4-PBS

Print datasheet

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

验证数据展示

  • WB analysis using 84580-4-RR (same clone as 84580-4-PBS)

    WB analysis using 84580-4-RR (same clone as 84580-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84580-4-RR (CD99 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84580-4-RR (same clone as 84580-4-PBS)

    IF Staining of Jurkat using 84580-4-RR (same clone as 84580-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD99 antibody (84580-4-RR, Clone: 241737H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

产品信息

84580-4-PBS targets CD99 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD99 molecule
Synonyms MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
Observed Molecular Weight28 kDa, 32 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).