• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84580-4-RR (same clone as 84580-4-PBS)

    WB analysis using 84580-4-RR (same clone as 84580-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84580-4-RR (CD99 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84580-4-RR (same clone as 84580-4-PBS)

    IF Staining of Jurkat using 84580-4-RR (same clone as 84580-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD99 antibody (84580-4-RR, Clone: 241737H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

CD99 Recombinant antibody, PBS Only

CD99 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84580-4-PBS

产品说明书

CloneNo. 241737H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84580-4-PBS targets CD99 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD99 molecule
别名 MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1
计算分子量 19 kDa, 16 kDa, 17 kDa
观测分子量28 kDa, 32 kDa
GenBank蛋白编号BC021620
基因名称 CD99
Gene ID (NCBI) 4267
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP14209
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).