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验证数据展示

  • WB analysis using 84590-1-RR

    WB analysis using 84590-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-1-RR (Phospho-CRKL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of K-562 using 84590-1-RR

    FC experiment of K-562 using 84590-1-RR

    1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR, Clone:240881B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-CRKL (Tyr207) Recombinant antibody

Phospho-CRKL (Tyr207) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84590-1-RR

产品说明书

CloneNo. 240881B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

p-CRKL (Tyr207), p-CRKL, CRKL (Tyr207), CrkL (phospho Y207), phospho CRKL (Tyr 207)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Positive FC (Intra) detected inλ phosphatase treated K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84590-1-RR targets Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-crk sarcoma virus CT10 oncogene homolog (avian)-like
别名 p-CRKL (Tyr207), p-CRKL, CRKL (Tyr207), CrkL (phospho Y207), phospho CRKL (Tyr 207)
计算分子量 34 kDa
观测分子量39 kDa
GenBank蛋白编号BC043500
基因名称 CRKL
Gene ID (NCBI) 1399
RRIDAB_3672086
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP46109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)

实验方案

Product Specific Protocols
WB protocol for Phospho-CRKL (Tyr207) antibody 84590-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols