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DCX Recombinant antibody

DCX Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241985H1

Cat no : 84595-4-RR

Print datasheet

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

验证数据展示

  • WB analysis of fetal human brain using 84595-4-RR

    WB analysis of fetal human brain using 84595-4-RR

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 84595-4-RR

    WB analysis of SH-SY5Y using 84595-4-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 84595-4-RR

    IHC staining of rat brain using 84595-4-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

经过测试的应用

Positive WB detected inSH-SY5Y cells, fetal human brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84595-4-RR targets DCX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen DCX fusion protein Ag4945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name doublecortin
Synonyms Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

实验方案

Product Specific Protocols
WB protocol for DCX antibody 84595-4-RRDownload protocol
IHC protocol for DCX antibody 84595-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols