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验证数据展示

  • WB analysis of fetal human brain using 84595-4-RR

    WB analysis of fetal human brain using 84595-4-RR

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 84595-4-RR

    WB analysis of SH-SY5Y using 84595-4-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 84595-4-RR

    IHC staining of rat brain using 84595-4-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

DCX Recombinant antibody

DCX Recombinant Antibody for WB, IHC, ELISA
Cat No. 84595-4-RR

产品说明书

CloneNo. 241985H1

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, fetal human brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84595-4-RR targets DCX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 DCX fusion protein Ag4945 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 doublecortin
别名 Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1
计算分子量 402 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC027925
基因名称 DCX
Gene ID (NCBI) 1641
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43602
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

实验方案

Product Specific Protocols
WB protocol for DCX antibody 84595-4-RRDownload protocol
IHC protocol for DCX antibody 84595-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols