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验证数据展示

  • WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

DCX Recombinant antibody, PBS Only

DCX Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84595-4-PBS

Print datasheet

CloneNo. 241985H1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84595-4-PBS targets DCX in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen DCX fusion protein Ag4945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name doublecortin
Synonyms Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43602
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.