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验证数据展示

  • WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84595-4-RR

    Affinity and Kinetic Characterization of 84595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

DCX Recombinant antibody, PBS Only

DCX Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84595-4-PBS

产品说明书

CloneNo. 241985H1

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84595-4-PBS targets DCX in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 DCX fusion protein Ag4945 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 doublecortin
别名 Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1
计算分子量 402 aa, 45 kDa
观测分子量 40 kDa
GenBank蛋白编号BC027925
基因名称 DCX
Gene ID (NCBI) 1641
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43602
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.