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GPAA1 Recombinant antibody

GPAA1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241591C4

Cat no : 84632-1-RR

Print datasheet

Synonyms

hGAA1, GPAA 1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1, 241591C4

验证数据展示

  • WB analysis using 84632-1-RR

    WB analysis using 84632-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84632-1-RR (GPAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84632-1-RR

    Affinity and Kinetic Characterization of 84632-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84632-1-RR against Human GPAA1 were performed. The affinity constant is 0.141 nM.

经过测试的应用

Positive WB detected inMKN-45 cells, HeLa cells, HepG2 cells, HuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84632-1-RR targets GPAA1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen GPAA1 fusion protein Ag0152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
Synonyms hGAA1, GPAA 1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1, 241591C4
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 60-68 kDa
GenBank Accession NumberBC004129
Gene Symbol GPAA1
Gene ID (NCBI) 8733
RRIDAB_3672093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).

实验方案

Product Specific Protocols
WB protocol for GPAA1 antibody 84632-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols