验证数据展示
经过测试的应用
| Positive WB detected in | MKN-45 cells, HeLa cells, HepG2 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84632-1-RR targets GPAA1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GPAA1 fusion protein Ag0152 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) |
| 别名 | hGAA1, GPAA 1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1, 241591C4 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 60-68 kDa |
| GenBank蛋白编号 | BC004129 |
| 基因名称 | GPAA1 |
| Gene ID (NCBI) | 8733 |
| RRID | AB_3672093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O43292 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPAA1 antibody 84632-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |