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验证数据展示

  • WB analysis using 84673-5-RR

    WB analysis using 84673-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84673-5-RR

    FC experiment of HeLa using 84673-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of SH-SY5Y using 84673-5-RR

    FC experiment of SH-SY5Y using 84673-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SSRP1 Recombinant antibody

SSRP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84673-5-RR
CloneNo. 242102H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84673-5-RR targets SSRP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SSRP1 fusion protein Ag8318 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name structure specific recognition protein 1
Synonyms FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit
Calculated Molecular Weight 81 kDa
Observed Molecular Weight86 kDa
GenBank Accession NumberBC005116
Gene Symbol SSRP1
Gene ID (NCBI) 6749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSRP1 antibody 84673-5-RRDownload protocol
FC protocol for SSRP1 antibody 84673-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols