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验证数据展示

  • FC experiment of HeLa using 84681-1-RR (same clone as 84681-1-PBS)

    FC experiment of HeLa using 84681-1-RR (same clone as 84681-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84681-1-RR (same clone as 84681-1-PBS)

    FC experiment of A549 using 84681-1-RR (same clone as 84681-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXM1 Recombinant antibody (84681-1-RR, Clone:242077A7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

    WB analysis using 84681-1-RR (same clone as 84681-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84681-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01500-3

    Sandwich ELISA standard curve of MP01500-3

    Sandwich ELISA standard curve of MP01500-3, Human FOXM1 Recombinant Matched Antibody Pair - PBS only. 84681-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3729. 84681-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

  • Sample test of MP01500-3

    Sample test of MP01500-3

    The mean FOXM1 concentration was determined to be 3.3 ng/mL in NCI-H1299 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01500-1

    Cytometric bead array standard curve of MP01500-1

    Cytometric bead array standard curve of MP01500-1, FOXM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84681-1-PBS. Detection antibody: 84681-2-PBS. Standard: Ag3729. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01500-2

    Cytometric bead array standard curve of MP01500-2

    Cytometric bead array standard curve of MP01500-2, FOXM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84681-1-PBS. Detection antibody: 84681-3-PBS. Standard: Ag3729. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84681-1-RR

    Affinity and Kinetic Characterization of 84681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

FOXM1 Recombinant antibody, PBS Only (Capture/Detector)

FOXM1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84681-1-PBS

产品说明书

CloneNo. 242077A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84681-1-PBS targets FOXM1 as part of a matched antibody pair:

MP01500-1: 84681-1-PBS capture and 84681-2-PBS detection (validated in Cytometric bead array)

MP01500-2: 84681-1-PBS capture and 84681-3-PBS detection (validated in Cytometric bead array)

MP01500-3: 84681-4-PBS capture and 84681-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 FOXM1 fusion protein Ag3729 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box M 1
别名 HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16
计算分子量 83 kDa
观测分子量 84-110 kDa
GenBank蛋白编号BC006192
基因名称 FOXM1
Gene ID (NCBI) 2305
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08050
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly as5sociated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).