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验证数据展示

  • WB analysis of SH-SY5Y using 84690-1-RR

    WB analysis of SH-SY5Y using 84690-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84690-1-RR

    WB analysis using 84690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84690-1-RR

    Affinity and Kinetic Characterization of 84690-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84690-1-RR against Human NRCAM were performed. The affinity constant is below 1 pM.

NRCAM Recombinant antibody

NRCAM Recombinant Antibody for WB, ELISA
Cat No. 84690-1-RR

产品说明书

CloneNo. 241862G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343, hBravo

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84690-1-RR targets NRCAM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NRCAM fusion protein Ag16237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neuronal cell adhesion molecule
别名 Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343, hBravo
计算分子量 1304 aa, 144 kDa
观测分子量144 kDa
GenBank蛋白编号BC098401
基因名称 NRCAM
Gene ID (NCBI) 4897
RRIDAB_3672106
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92823
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

实验方案

Product Specific Protocols
WB protocol for NRCAM antibody 84690-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols