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CYP51A1 Recombinant antibody

CYP51A1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242166G9

Cat no : 84705-4-RR

Print datasheet

Synonyms

Cytochrome P450 14DM, CYPLI, CYPL1, CYP51, CP51

验证数据展示

  • WB analysis using 84705-4-RR

    WB analysis using 84705-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84705-4-RR (CYP51A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84705-4-RR

    Affinity and Kinetic Characterization of 84705-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84705-4-RR against Human CYP51A1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, HEK-293 cells, RAW 264.7 cells, mouse testis tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84705-4-RR targets CYP51A1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP51A1 fusion protein Ag4235 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome P450, family 51, subfamily A, polypeptide 1
Synonyms Cytochrome P450 14DM, CYPLI, CYPL1, CYP51, CP51
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC032322
Gene Symbol CYP51A1
Gene ID (NCBI) 1595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16850
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).

实验方案

Product Specific Protocols
WB protocol for CYP51A1 antibody 84705-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols