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WASP Recombinant antibody

WASP Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242074E4

Cat no : 84714-2-RR

Print datasheet

Synonyms

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4

验证数据展示

  • WB analysis using 84714-2-RR

    WB analysis using 84714-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84714-2-RR (WASP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84714-2-RR

    Affinity and Kinetic Characterization of 84714-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84714-2-RR against Human WASP were performed. The affinity constant is 1.88 nM.

经过测试的应用

Positive WB detected inJurkat cells, TF-1 cells, HL-60 cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy WAS Ready-To-Use IHC Kit. WAS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84714-2-RR targets WASP in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen WASP fusion protein Ag1444 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Synonyms Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC012738
Gene Symbol WASP
Gene ID (NCBI) 7454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

实验方案

Product Specific Protocols
WB protocol for WASP antibody 84714-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols